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Mouse anti-Human IgA2 (IgA2(m)2) Antibody (Biotin)

ELISA, WB, ICC, IHC (p), DB Monoclonal NI 194-3 (A89-040) IgG1 Biotin
Catalog No. ABIN457865
  • Target See all IgA2 products
    IgA2
    Binding Specificity
    • 4
    • 4
    • 1
    IgA2(m)2
    Reactivity
    Human
    Host
    • 11
    • 2
    Mouse
    Clonality
    • 11
    • 2
    Monoclonal
    Conjugate
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Biotin
    Application
    • 10
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
    Specificity
    Biotin-conjugated purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific
    Cross-Reactivity (Details)
    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
    Characteristics
    The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA2(m)2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assayrelated characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:200 upwards.
    Purification
    Purified
    Immunogen
    Highly purified monoclonal IgA2(m)2 isolated from human serum.
    Clone
    NI 194-3 (A89-040)
    Isotype
    IgG1
  • Application Notes
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
    Buffer
    Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
    Preservative
    Without preservative
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
  • Target
    IgA2
    Alternative Name
    IgA22 (IgA2 Products)
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