Goat anti-Human IgG (Chain kappa), (Chain lambda), (Fab Region), (Free & Bound) Antibody (TRITC)

Catalog No. ABIN458071

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Chain kappa, Chain lambda, Fab Region, Free & Bound

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: TRITC

Application: Immunofluorescence (IF)

Specificity: The antiserum is reacting with to the Fab subunits of the IgG, IgA, IgM and other Ig classes of both light chain type, with their Fab or F(ab')2 subunits and with free immunoglobulin light chains of kappa and lambda type.

Characteristics: Tetramethylrhodamine isothiocyanate-conjugated F(ab')2 fragments IgG antibodies of poly-clonal goat antiserum to human Fab IgG
Fluorochrome/IgG protein molar ratio (F/P) is approximately 2.2.

Purification: No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Purified Fab prepared from normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen , and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifu-gation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody