Amyloid beta (Abeta) (Oligomer-Specific) antibody

Antigen: Amyloid beta (Abeta)

Synonyms: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, CTFgamma, aaa, ad1, pn2, abpp, appi, cvap, abeta, MGC88882, ctfgamma, APP

Epitope: Oligomer-Specific

Clonality: Monoclonal (OMAB)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN459388

Additional Information

Characteristics: OMAB antibody is a versatile tool within research of Alzheimer’s disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Aβ oligomers.

Alternative name: Amyloid beta oligomer-specific

Immunogen: partly aggregated, recombinant peptide corresponding to the human Abeta (1-40). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V

Format: Lyophilized

Isotype: IgM  (Matching secondary antibodies)

Clone: OMAB

Description: Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

Specificity: Confirmed reactivity: Abeta oligomers

Molecular Weight: 4.5 kDa

Application Details

Application Notes: coating antibody at 2 µg/ml (ELISA), 1: 500 (IHC)

Concentration: For reconstitution add 100 µl of sterile water.

Purity: Affinity Purified

Buffer: OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.

Storage: store lyophilized/reconstituted at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Images

Abeta oligomer-specific antibody was adsorbed to Nunc-Immuno MaxiSorp plates (Nunc, Roskilde, Denmark) at 2 ug/ml in PBS. 1 ml of a 10 uM Aβ(1-42) sample containing a small fraction of Aβ-oligomers was separated using a superdex G75 (10/30) column. Aβ-fractions collected from the SEC were allowed to bind to OMAB plates for 20 minutes at 0°C. All fractions were analyzed and bound Aβ was detected using a polyclonal rabbit anti-Aβ antibody (ABIN249343) at a 1:1000 dilution followed by an anti-rabbit HRP-conjugated secondary antibody at a 1:5000 dilution (GE healthcare). ECBlue (Medicago, Uppsala, Sweden) was used as a substrate for HRP and the signal was detected by measuring the absorbance at 450 nm. Blocking solution and antibody-dilutions were made with 5% Non-fat dry milk in PBST and all washes were performed with PBS containing 0.1% Tween-20 (PBST).

10 µm of coronal sections from fresh-frozen transgenic mouse brain mutant (A) and wild type (B). Post-fixation in 4% formaldehyde solution, 5 min. OMAB antibody diluted 1:500, incubation at 4ºC ON. Mouse on mouse HRP-Polymer kit according to company instructions. Biocare Medical: BC-MM510 (Histolab) DAB substrate kit for peroxidase. Vector Laboratories: SK-4100 (ImmunKemi) Counterstained with Mayers HTX.

Publications

Publications: Lindhagen-Persson, Bra?nnstra?m, Vestling et al.: "Amyloid-β oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies." in: PLoS ONE, Vol. 5, Issue 11, pp. e13928, 2010 (PubMed).