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beta Amyloid antibody (C-Term)

Abeta Reactivity: Human, Mouse, Rat WB, ELISA, IF Host: Mouse Monoclonal 9E8-1A3 unconjugated
Catalog No. ABIN5508784
  • Target See all beta Amyloid (Abeta) Antibodies
    beta Amyloid (Abeta) (Amyloid beta (Abeta))
    Binding Specificity
    • 18
    • 17
    • 11
    • 6
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 69
    • 21
    • 14
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 41
    • 26
    • 3
    • 2
    Mouse
    Clonality
    • 37
    • 29
    • 1
    Monoclonal
    Conjugate
    • 62
    • 5
    • 2
    • 1
    • 1
    • 1
    This beta Amyloid antibody is un-conjugated
    Application
    • 52
    • 49
    • 29
    • 16
    • 15
    • 13
    • 9
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Specificity
    A-beta 1-40, A-beta 1-42, A-beta 1-43 and APP
    Cross-Reactivity (Details)
    Human, mouse, rat (other species not tested)
    Purification
    Protein G affinity purification
    Immunogen
    Synthetic peptide derived from Human Abeta 1-42 protein
    Clone
    9E8-1A3
    Isotype
    IgG1
  • Application Notes
    ELISA: 1:40,000, WB: 1:4,000, IF: 1:2,000
    Restrictions
    For Research Use only
  • Validation #104425 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #104425
    Date
    05/12/2022
    Antigen
    beta Amyloid
    Lot Number
    190522A
    Method validated
    Immunohistochemistry
    Positive Control

    Adult (24 months) mouse brain

    3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde

    Negative Control

    We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

    Notes

    The beta amyloid antibody (Amyloid beta) (C-Term) ABIN5508784 works in IHC-P, especially at higher concentrations (1:200 and 1:500), and without the use of any antigen retrieval treatment.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN5508784
    Secondary Antibody
    goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
    Full Protocol
    • Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
    • Wash, dehydrate, and embed samples in paraffin wax.
    • Wash several times with 0.01 M PBS.
    • Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
    • After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
    • Incubate sections with primary anti-beta amyloid (C-Term) (antibodies Online, ABIN5508784, lot 190522A) diluted 1:200, 1:500, 1:1000, and 1:2000 in PBS-BSA-PLL ON at RT.
    • Wash sections 3x 5 min with 0.01 M PBS.
    • Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
    • Wash sections 3x 5 min with 0.01M PBS.
    • Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    • Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
    Experimental Notes

    Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min(95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.

  • Validation #104493 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #104493
    Date
    03/15/2023
    Antigen
    Abeta
    Lot Number
    190522A
    Method validated
    Immunohistochemistry
    Positive Control

    3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde

    Negative Control

    One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.

    Notes

    The beta Amyloid antibody (Amyloid beta) (C-Term) ABIN5508784 works in IHC-P, especially at higher concentrations (1:200 and 1:500). Tyramide amplification strongly enhances the positive signal.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN5508784
    Secondary Antibody
    poly-HRP conjugated goat anti-rabbit antibody
    Full Protocol
    • Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
    • Wash, dehydrate, and embed samples in paraffin wax.
    • Wash several times with 0.01 M PBS.
    • Cut intestines and brain with a microtome into 6 µm sections and mount on glass slides.
    • After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
    • Incubate sections with primary anti-beta amyloid (N-Term) (antibodies-online, ABIN5508784, lot 190522A) diluted 1:200, 1:500, 1:1000, and 1:2000 in 0.1 M PBS-BSA-PLL ON at RT.
    • Wash sections 3x 5 min with 0.01 M PBS.
    • Incubate sections with poly-HRP conjugated goat anti-rabbit antibody for 1 h at RT.
    • Wash sections 3x 5 min with 0.01 M PBS.
    • Incubate sections Tyramide working solution (for 5 sections: 100X Tyramide stock solution 5 μL, 100X H2O2 solution 5 μL, and 1X Reaction buffer 500 μL; Thermo Fisher Scientific, B40915, lot 2086865) for 10 min.
    • Stop the reaction with the Reaction Stop Reagent working solution.
    • Wash sections 3x 5 min with 0.01M PBS.
    • Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    • Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
    Experimental Notes
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, pH 7.4
    Preservative
    Without preservative
    Handling Advice
    Avoid freezing/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C to -80°C.
  • Ordóñez-Gutiérrez, Posado-Fernández, Ahmadvand, Lettiero, Wu, Antón, Flores, Moghimi, Wandosell: "ImmunoPEGliposome-mediated reduction of blood and brain amyloid levels in a mouse model of Alzheimer's disease is restricted to aged animals." in: Biomaterials, Vol. 112, pp. 141-152, (2016) (PubMed).

    Markoutsa, Papadia, Clemente, Flores, Antimisiaris: "Anti-Aβ-MAb and dually decorated nanoliposomes: effect of Aβ1-42 peptides on interaction with hCMEC/D3 cells." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 81, Issue 1, pp. 49-56, (2012) (PubMed).

    Le Droumaguet, Nicolas, Brambilla, Mura, Maksimenko, De Kimpe, Salvati, Zona, Airoldi, Canovi, Gobbi, Magali, La Ferla, Nicotra, Scheper, Flores, Masserini, Andrieux, Couvreur: "Versatile and efficient targeting using a single nanoparticulate platform: application to cancer and Alzheimer's disease." in: ACS nano, Vol. 6, Issue 7, pp. 5866-79, (2012) (PubMed).

    Canovi, Markoutsa, Lazar, Pampalakis, Clemente, Re, Sesana, Masserini, Salmona, Duyckaerts, Flores, Gobbi, Antimisiaris: "The binding affinity of anti-Aβ1-42 MAb-decorated nanoliposomes to Aβ1-42 peptides in vitro and to amyloid deposits in post-mortem tissue." in: Biomaterials, Vol. 32, Issue 23, pp. 5489-97, (2011) (PubMed).

  • Target
    beta Amyloid (Abeta) (Amyloid beta (Abeta))
    Alternative Name
    Amyloid beta (Abeta Products)
    Synonyms
    AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN-II antibody, PN2 antibody, aaa antibody, abeta antibody, abpp antibody, ad1 antibody, appi antibody, ctfgamma antibody, cvap antibody, pn2 antibody, Abeta antibody, Abpp antibody, Adap antibody, Ag antibody, Cvap antibody, E030013M08Rik antibody, betaApp antibody, amyloid beta precursor protein antibody, amyloid beta (A4) precursor protein antibody, APP antibody, app antibody, App antibody
    Background
    Amyloid beta (Aβ or Abeta) is a peptide of 36 - 43 amino acids derived from the cleavage of APP (Amyloid Precursor Protein) by β- and γ-secretases. It is involved in Alzheimer´s disease, among other severe neurological disorders. It has been proved that the Aβ could be a crucial target for these important diseases treatment.
    Pathways
    Inflammasome
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