ZNF165 antibody (AA 216-265)

Catalog No. ABIN6741560

Product Details anti-ZNF165 Antibody

Target: ZNF165 (Zinc Finger Protein 165 (ZNF165))

Binding Specificity: AA 216-265

Reactivity: Human, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF165 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ZNF165

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human ZNF165 (P49910, NP_003438). Percent identity by BLAST analysis: Human, Monkey (100%), Gorilla, Gibbon (92%), Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ZNF165

Target: ZNF165 (Zinc Finger Protein 165 (ZNF165))

Alternative Name: ZNF165 (ZNF165 Products)

Synonyms: CT53 antibody, LD65 antibody, ZSCAN7 antibody, ZSCAN16 antibody, MGC140342 antibody, zinc finger protein 165 antibody, ZNF165 antibody

Background: Name/Gene ID: ZNF165
Family: Zinc Finger

Synonyms: ZNF165, Cancer/testis antigen 53, CT53, LD65, ZPF165, Zinc finger protein 165, ZSCAN7

Gene ID: 7718

NCBI Accession: NP_003438

UniProt: P49910