APOC3 antibody

Catalog No. ABIN462267

The Goat Polyclonal anti-APOC3 antibody (ABIN462267) specifically detects APOC3 in WB, ELISA, IHC, IP and IHC (p). The antibody is reactive with Human samples.

Quick Overview for APOC3 antibody (ABIN462267)

Target: APOC3 (Apolipoprotein C-III (APOC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOC3 Antibody

Specificity: Recognizes human apolipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apolipoprotein. Non-specific cross-reactivity of anti-apolipoprotein antibodies with other human serum proteins is negligible.

Purification: Immunoaffinity purified

Immunogen: Synthetic full-length Apolipoprotein Type C-III.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:10000), IHC, IHC-P (2.5 μg/mL), IP (1:100), WB (1:500 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: 1:500-1:5000. ELISA: 1:5000-1:10000. Immunoprecipitation: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APOC3

Target: APOC3 (Apolipoprotein C-III (APOC3))

Alternative Name: APOC3 / Apolipoprotein C III

Background: Name/Gene ID: APOC3

Synonyms: APOC3, Apo-CIII, Apolipoprotein C3, APOCIII, HALP2, ApoC-III, Apolipoprotein C-III

Gene ID: 345

UniProt: P02656

Pathways: Carbohydrate Homeostasis, Lipid Metabolism