PDGFRB antibody

Catalog No. ABIN462353

The Mouse Monoclonal anti-PDGFRB antibody is suitable to detect PDGFRB in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IHC (p).

Quick Overview for PDGFRB antibody (ABIN462353)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFRB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 42G12

Product Details anti-PDGFRB Antibody

Specificity: Human PDGFR beta.

Purification: Protein G purified

Immunogen: Recombinant human PDGF receptor beta protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: PDGFRB / PDGFR Beta

Background: Name/Gene ID: PDGFRB
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRB, CD140B, Beta pdfgr, Beta type PDGFR, BPDGFR, CD140b antigen, IBGC4, JTK12, PDGF-beta R, PDGF-R-beta, PDGFR, PDGFRbeta, PDGF receptor beta, PDGF-R, PDGFbetaR, PDGFR Beta, PDGFR-1, PDGFR-beta, BetaPDGFR, Pdgf beta-receptor, PDGFR1, PDGFRB/CEV14 fusion gene

Gene ID: 5159

UniProt: P09619

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling