Cytokeratin 18 antibody

Catalog No. ABIN462411

The Mouse Monoclonal anti-Cytokeratin 18 antibody (Clone DA-7) (ABIN462411) specifically detects Cytokeratin 18 in WB, IHC, ELISA, IHC (p), ICC, IP and InhibAb. The antibody is reactive with Human samples.

Quick Overview for Cytokeratin 18 antibody (ABIN462411)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Blocking Antibody (InhibAb)

Clone: DA-7

Product Details anti-Cytokeratin 18 Antibody

Specificity: Reacts with Cytokeratin 18 (45 kD). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

No Cross-Reactivity: Mouse (Murine), Cow (Bovine), Sheep (Ovine), Dog (Canine), Rat (Rattus), Hamster, Pig (Porcine)

Purification: Protein A purified

Immunogen: Human breast carcinoma cell line PMC-42.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-P (10 μg/mL), Inhb, IP (1 - 2 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody,

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18 / CK18 / Cytokeratin 18

Background: Name/Gene ID: KRT18
Family: Keratin

Synonyms: KRT18, CK-18, Cytokeratin 18, Cytokeratin-18, K18, Keratin 18, CYK18, Keratin-18

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis