CARS antibody (AA 756-805)

Catalog No. ABIN6740660

Product Details anti-CARS Antibody

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Binding Specificity: AA 756-805

Reactivity: Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARS antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human CARS

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat (100%) Dog, Bovine, Guinea pig, Chicken (92%) Mouse (85%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa756-805 of human CARS (Q5HYE4, NP_001014437). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Rat (100%), Galago, Dog, Bovine, Horse, Guinea pig, Chicken, Lizard (92%), Turkey (91%), Slime mold (90%), Mouse, Drosophila (85%), Salmon, Zebrafish (84%), Xenopus, Stickleback (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CARS

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Alternative Name: CARS1 / CARS (CARS Products)

Synonyms: sb:cb71 antibody, CARS antibody, BcDNA:LD21177 antibody, CG8431 antibody, CRS antibody, CysRS antibody, Dmel\\CG8431 antibody, BA0089 antibody, DDBDRAFT_0215191 antibody, DDBDRAFT_0231320 antibody, DDB_0215191 antibody, DDB_0231320 antibody, DDBDRAFT_0187313 antibody, DDBDRAFT_0231318 antibody, DDB_0187313 antibody, DDB_0231318 antibody, T16B12.16 antibody, K15E6.3 antibody, K15E6_3 antibody, CARS1 antibody, CYSRS antibody, MGC:11246 antibody, CA3 antibody, cysteinyl-tRNA synthetase antibody, Cysteinyl-tRNA synthetase antibody, cysteine--tRNA ligase antibody, cysteine-tRNA ligase antibody, Cysteinyl-tRNA synthetase, class Ia family protein antibody, cysteinyl-tRNA synthetase CysS antibody, cysteinyl-tRNA synthetase S homeolog antibody, CARS antibody, cars antibody, CysRS antibody, cysS antibody, APH_RS02395 antibody, mcysS antibody, SYCO ARATH antibody, AT3G56300 antibody, AT5G38830 antibody, cars.S antibody, Cars antibody

Background: Name/Gene ID: CARS

Synonyms: CARS, Cysteinyl-tRNA synthetase, CYSRS, Cysteine translase, CARS1, MGC:11246

Gene ID: 833

NCBI Accession: NP_001014437

UniProt: P49589