HACE1 antibody (AA 792-841)

Catalog No. ABIN6740811

The Rabbit Polyclonal anti-HACE1 antibody (ABIN6740811) specifically detects HACE1 in WB. The antibody is reactive with Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Monkey and Pig samples.

Quick Overview for HACE1 antibody (AA 792-841) (ABIN6740811)

Target: HACE1 (HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HACE1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HACE1 Antibody

Binding Specificity: AA 792-841

Specificity: Human HACE1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Chicken, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa792-841 of human HACE1 (Q8IYU2, NP_065822). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Platypus (100%), Bat, Opossum (92%), Turkey, Zebra finch, Sparrow, Chicken, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HACE1

Target: HACE1 (HECT Domain and Ankyrin Repeat Containing, E3 Ubiquitin Protein Ligase 1 (HACE1))

Alternative Name: HACE1

Background: Name/Gene ID: HACE1

Synonyms: HACE1, HACE, KIAA1320

Gene ID: 57531

NCBI Accession: NP_065822

UniProt: Q8IYU2