NHE8 antibody (C-Term)

Catalog No. ABIN6740850

This Rabbit Polyclonal antibody specifically detects NHE8 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Monkey, Chicken and Xenopus laevis.

Quick Overview for NHE8 antibody (C-Term) (ABIN6740850)

Target: NHE8 (SLC9A8) (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 8 (SLC9A8))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Monkey, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHE8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NHE8 Antibody

Binding Specificity: C-Term

Specificity: Human SLC9A8

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea pig, Chicken (100%) Bovine, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SLC9A8 (Q2M1U9, NP_056081). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Xenopus (100%), Bovine, Bat, Lizard, Zebrafish (92%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NHE8

Target: NHE8 (SLC9A8) (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 8 (SLC9A8))

Alternative Name: SLC9A8

Background: Name/Gene ID: SLC9A8
Subfamily: Monovalent cation:proton antiporter-1
Family: Transporter

Synonyms: SLC9A8, KIAA0939, Na(+)/H(+) exchanger 8, NHE8, NHE-8, Sodium/hydrogen exchanger 8

Gene ID: 23315

NCBI Accession: NP_056081

UniProt: Q9Y2E8

Pathways: Proton Transport