NHE8 antibody (AA 420-500)

Catalog No. ABIN7220690

The Rabbit Polyclonal anti-NHE8 antibody (ABIN7220690) specifically detects NHE8 in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NHE8 antibody (AA 420-500) (ABIN7220690)

Target: NHE8 (SLC9A8) (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 8 (SLC9A8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHE8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NHE8 Antibody

Binding Specificity: AA 420-500

Purpose: NHE-8 Polyclonal Antibody

Specificity: NHE-8 Polyclonal Antibody detects endogenous levels of NHE-8 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human NHE-8 at AA range: 420-500

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NHE8

Target: NHE8 (SLC9A8) (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 8 (SLC9A8))

Alternative Name: NHE-8

Background: Rabbit Anti-NHE-8 Polyclonal Antibody,SLC9A8, KIAA0939, NHE8, Sodium/hydrogen exchanger 8, Na(+)/H(+) exchanger 8, NHE-8, Solute carrier family 9 member 8,Sodium-hydrogen exchangers (NHEs), such as SLC9A8, are integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHEs have multiple functions, including intracellular pH homeostasis, cell volume regulation, and electroneutral NaCl absorption in epithelia (Xu et al. 2008 [PubMed 18209477]).,Sodium hydrogen exchanger 8

Molecular Weight: observerd band 65kDa

Gene ID: 23315

UniProt: Q9Y2E8

Pathways: Proton Transport