TNFSF15 antibody (Biotin)

Catalog No. ABIN464857

This Rabbit Polyclonal antibody specifically detects TNFSF15 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for TNFSF15 antibody (Biotin) (ABIN464857)

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFSF15 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-TNFSF15 Antibody (Biotin)

Specificity: Human TL-1A

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TL-1A.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hTL-1A by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTL-1A. To detect hTL-1A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TL-1A (LS-B7475) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTL-1A. Western Blot: To detect hTL-1A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTL-1A is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TNFSF15

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: TNFSF15 / TL1A / VEGI

Background: Name/Gene ID: TNFSF15
Family: TNF

Synonyms: TNFSF15, TL1, TL1A, TNF ligand-related molecule 1, TNF superfamily ligand TL1A, VEGI192A, VEGI

Gene ID: 9966

UniProt: O95150

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy