CXCL1 antibody

Catalog No. ABIN464980

This Rabbit Polyclonal antibody specifically detects CXCL1 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CXCL1 antibody (ABIN464980)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL1 Antibody

Specificity: Human GRO/MGSA

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human GRO/MGSA.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (1.5 - 3 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 μg/mL-0.150 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human GRO/MGSA (100 ng/mL), a concentration of 1.5-3 μg/mL of this antibody is required. ELISA: To detect Human GRO/MGSA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GRO/MGSA (LS-C104350) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human GRO/MGSA. Western Blot: To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: GRO / CXCL1

Background: Name/Gene ID: CXCL1
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL1, C-X-C motif chemokine 1, Fibroblast secretory protein, FSP, GRO1, GROa, Growth-regulated alpha protein, GRO-alpha(1-73), MGSA, MGSA-a, Small inducible cytokine b1, GRO, MGSA alpha, NAP-3, SCYB1

Gene ID: 2919

UniProt: P09341

Pathways: Autophagy