LGALS1/Galectin 1 antibody (Biotin)

Catalog No. ABIN465024

The Biotin-conjugated Rabbit Polyclonal anti-LGALS1/Galectin 1 antibody (ABIN465024) specifically detects LGALS1/Galectin 1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for LGALS1/Galectin 1 antibody (Biotin) (ABIN465024)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGALS1/Galectin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-LGALS1/Galectin 1 Antibody (Biotin)

Specificity: Human Galectin-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Galectin-1.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hGalectin-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-1. To detect hGalectin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Galectin-1 (LS-B7436) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-1. Western Blot: To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: LGALS1 / Galectin 1

Background: Name/Gene ID: LGALS1

Synonyms: LGALS1, 14 kDa lectin, 14 kDa laminin-binding protein, 14 kd lectin, GAL1, Galectin 1, Galectin-1, HBL, Lactose-binding lectin 1, Galaptin, GBP, HLBP14, HPL, S-Lac lectin 1, Gal-1

Gene ID: 3956

UniProt: P09382

Pathways: Carbohydrate Homeostasis