Midkine antibody (Biotin)

Catalog No. ABIN465383

The Rabbit Polyclonal anti-Midkine antibody is suitable to detect Midkine in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Midkine antibody (Biotin) (ABIN465383)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midkine antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Midkine Antibody (Biotin)

Specificity: Human Midkine

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Midkine.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hMidkine by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMidkine. To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Midkine (LS-B8221) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: MDK / Midkine

Background: Name/Gene ID: MDK

Synonyms: MDK, ARAP, MK, MK1, NEGF2, Midkine, Retinoic acid inducible factor

Gene ID: 4192

UniProt: P21741

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development