Thrombopoietin antibody

Catalog No. ABIN465842

This Goat Polyclonal antibody specifically detects Thrombopoietin in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for Thrombopoietin antibody (ABIN465842)

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Thrombopoietin Antibody

Specificity: Human TPO

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TPO.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (4.2 - 6.3 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hTPO (50 ng/mL), a concentration of 05-08 μg/mL of this antibody is required. ELISA: To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (LS-C104660) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: THPO / TPO / Thrombopoietin

Background: Name/Gene ID: THPO
Family: Hormone

Synonyms: THPO, C-mpl ligand, MGDF, MPL ligand, THCYT1, Thrombopoietin nirs variant 1, MKCSF, MPLLG, Thrombopoietin

Gene ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity