Separase antibody (pSer801)

Catalog No. ABIN481852

This Rabbit Polyclonal antibody specifically detects Separase in ELISA, IHC, WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for Separase antibody (pSer801) (ABIN481852)

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Separase antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Separase Antibody

Binding Specificity: AA 767-816, pSer801

Specificity:

SEPARASE (Phospho-Ser801) Antibody detects endogenous levels of SEPARASE only when phosphorylated at Ser801.

PhosphorylationH:S801 M:S1189

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Comment:

Unigene-Number: Hs.248141 (NCBI Gene Symbol: ESPL1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Alternative Name: SEPARASE

Background:

Synonyms: ESP1, SEPARIN, EXTRA SPINDLE POLES-LIKE 1, extra spindle poles like 1 (S. cerevisiae)

NCBI Gene Symbol: ESPL1

Molecular Weight: 233 kDa

Gene ID: 9700

OMIM: 600937

UniProt: Q14674