LAT antibody (pTyr200)

Catalog No. ABIN482049

The Rabbit Polyclonal anti-LAT antibody (ABIN482049) specifically detects LAT in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LAT antibody (pTyr200) (ABIN482049)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LAT Antibody

Binding Specificity: AA 171-220, pTyr200

Specificity:

LAT (Phospho-Tyr171) Antibody detects endogenous levels of LAT only when phosphorylated at Tyr171.

PhosphorylationH:Y200 M:Y175 R:Y175

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human LAT around the phosphorylation site of Tyr171.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:1000

Comment:

Unigene-Number: Hs.632179 (NCBI Gene Symbol: LAT)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT

Background:

Synonyms: 36 kDa phospho-tyrosine adaptor protein, Linker for activation of T cells, p36-38, pp36

NCBI Gene Symbol: LAT

Molecular Weight: 24 kDa

Gene ID: 27040

OMIM: 602354

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway