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SMAD2 antibody (pThr220)

The Rabbit Polyclonal anti-SMAD2 antibody (ABIN482241) specifically detects SMAD2 in ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

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Catalog No. ABIN482241

$225.50

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Quick Overview for SMAD2 antibody (pThr220) (ABIN482241)

Target

See all SMAD2 Antibodies

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This SMAD2 antibody is un-conjugated

Application

ELISA, Immunofluorescence (IF)

Discover our top product SMAD2 Primary Antibody

Binding Specificity
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AA 186-235, pThr220

Specificity

Smad2 (Phospho-Thr220) Antibody detects endogenous levels of Smad2 only when phosphorylated at Thr220.
PhosphorylationH:T220 M:T220 R:T220

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220.

Isotype

IgG
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3G9 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (C-Term) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibody
SMAD2 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, Co-IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 4D10 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer467) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pThr183), (pThr221) antibody
SMAD2 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 83-264) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (N-Term) antibody
SMAD2 Reactivity: Human WB, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 2D7 unconjugated

Application Notes

IF: 1:100~1:500 ELISA: 1:1000

Comment

Unigene-Number: Hs.12253, Hs.705764 (NCBI Gene Symbol: SMAD2)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Target

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name

Smad2

Background

Synonyms: JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2
NCBI Gene Symbol: SMAD2

Molecular Weight

52 kDa

Gene ID

4087

OMIM

601366

UniProt

Q15796

Pathways

Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus