HIRA antibody (pThr555)

Catalog No. ABIN482264

The Rabbit Polyclonal anti-HIRA antibody is suitable to detect HIRA in samples from Human and Mouse. It has been validated for ELISA and IF.

Quick Overview for HIRA antibody (pThr555) (ABIN482264)

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIRA antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-HIRA Antibody

Binding Specificity: AA 521-570, pThr555

Specificity:

HIRA (Phospho-Thr555) Antibody detects endogenous levels of HIRA only when phosphorylated at Thr555.

PhosphorylationH:T555 M:T554

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human HIRA around the phosphorylation site of Thr555.

Isotype: IgG

Application Details

Application Notes: IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.474206 (NCBI Gene Symbol: HIRA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: HIRA

Background:

Synonyms: DGCR1, TUP1 like enhancer of split protein 1, TUPLE1

NCBI Gene Symbol: HIRA

Molecular Weight: 111 kDa

Gene ID: 7290

OMIM: 600237

UniProt: P54198

Pathways: Chromatin Binding