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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody

Antigen

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)

Synonyms Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B, MGC139723
Epitope
Alternatives

pThr34

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
Catalog no. ABIN482426
Quantity 50µg  (1 mg/ml)
Price 150.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name DARPP-32 (Phospho-Thr34)
Gene ID 84152 (NCBI Gene Symbol: PPP1R1B)
Swiss-Prot Q9UD71
Immunogen The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34.
Format Liquid
Isotype IgG  (Matching secondary antibodies)
Description Synonyms: Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
Specificity DARPP-32 (Phospho-Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine34.
Molecular Weight 22 kDa
Comments

OMIM-Number: 604399, Unigene-Number: Hs.286192

Application Details

Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Concentration 1 mg/ml
Purity Greater 95%
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C/1 year
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
Hosts Rabbit (48), Mouse (4)
Reactivities Human (43), Rat (Rattus) (37), Mouse (Murine) (34), Cow (Bovine) (12), Dog (Canine) (7), Chicken (6), Rabbit (3), Monkey (2), Xenopus laevis (2), Pig (Porcine) (1)
Applications Western Blotting (WB) (32), Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), ELISA (6), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pThr75 (8), pThr34 (5), Ab-34 (1), Ab-75 (1), N-Term (1)