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Antibodies
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anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) a...
Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody
| Antigen |
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| Synonyms |
Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B, MGC139723 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
Western Blotting (WB) (32), Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), ELISA (6), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN482426 |
| Quantity |
50µg (1 mg/ml) |
| Price |
150.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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DARPP-32 (Phospho-Thr34)
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Gene ID
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84152 (NCBI Gene Symbol: PPP1R1B)
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Swiss-Prot
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Q9UD71
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Immunogen
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The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34.
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Format
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Liquid
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Synonyms: Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
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Specificity
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DARPP-32 (Phospho-Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine34.
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Molecular Weight
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22 kDa
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Comments
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OMIM-Number: 604399, Unigene-Number: Hs.286192
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
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Concentration
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1 mg/ml
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Purity
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Greater 95%
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
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Buffer
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Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C/1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
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Hosts
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Rabbit (48), Mouse (4)
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Reactivities
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Human (43), Rat (Rattus) (37), Mouse (Murine) (34), Cow (Bovine) (12), Dog (Canine) (7), Chicken (6), Rabbit (3), Monkey (2), Xenopus laevis (2), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (32), Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), ELISA (6), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr75 (8), pThr34 (5), Ab-34 (1), Ab-75 (1), N-Term (1)
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