DARPP32 antibody (pThr34)

Catalog No. ABIN482426

The Rabbit Polyclonal anti-DARPP32 antibody is suitable to detect DARPP32 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for DARPP32 antibody (pThr34) (ABIN482426)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DARPP32 Antibody

Binding Specificity: AA 18-67, pThr34

Specificity:

DARPP-32 (Phospho-Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at Thr34.

PhosphorylationH:T34 M:T34 R:T34

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.286192 (NCBI Gene Symbol: PPP1R1B)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP-32

Background:

Synonyms: Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B

NCBI Gene Symbol: PPP1R1B

Molecular Weight: 22 kDa

Gene ID: 84152

OMIM: 604399

UniProt: Q9UD71