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Heat Shock 27kDa Protein 1 (HSPB1) (pSer82) antibody
| Antigen | Heat Shock 27kDa Protein 1 (HSPB1) |
| Synonyms |
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, M ...
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| Epitope |
 Alternatives pSer82 |
| Clonality | Polyclonal |
| Host |
Alternatives Rabbit |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB), ELISA
|
| Catalog no. | ABIN482480 |
| Quantity | 50µg (1 mg/ml) |
| Price | 150.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | HSP27 (Phospho-Ser82) |
| Gene ID | 3315 (NCBI Gene Symbol: HSPB1) |
| Swiss-Prot | P04792 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser82. |
| Format | Liquid |
| Isotype | IgG (Matching secondary antibodies) |
| Description | Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, Heat shock 27 kDa protein, HSP 27, HSP25, HSPB1, P25, SRP27, Stress-responsive protein 27 |
| Specificity | HSP27 (Phospho-Ser82) Antibody detects endogenous levels of HSP27 only when phosphorylated at serine82. |
| Molecular Weight | 22 kDa |
| Comments |
OMIM-Number: 602195 / 606595 / 608634, Unigene-Number: Hs.520973 |
| Synonyms | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259 |
Application Details
| Application Notes | WB: 1:500~1:1000 ELISA: 1:4000 |
| Concentration | 1 mg/ml |
| Purity | Greater 95% |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage | Store at -20°C/1 year |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "Heat Shock 27kDa Protein 1 (HSPB1)", type "Antibodies"
| Hosts | Rabbit (126), Mouse (73), Chicken (1) |
| Reactivities | Human (188), Rat (Rattus) (27), Mouse (Murine) (21), Dog (Canine) (10), Chicken (6), Cow (Bovine) (6), Monkey (5), Mammalian (4), Cat (Feline) (3), Fish (3), Goat (3), Guinea Pig (3), Hamster (3), Pig (Porcine) (3), Horse (Equine) (2) |
| Applications | Western Blotting (WB) (184), Immunohistochemistry (IHC) (71), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), ELISA (42), Immunofluorescence (IF) (38), Immunoprecipitation (IP) (37), Enzyme Immunoassay (EIA) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunocytochemistry (ICC) (15), Flow Cytometry (FACS) (13), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (2), ELISA (Detection) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (7), FITC (7), DY488 (1), PE (1) |
| Epitopes | pSer78 (12), pSer82 (12), pSer15 (11), p (4), S78 (2), pSer83 (2), Center (1), S15 (1), S82 (1), S83 (1), pS83 (1), pSer78/82 (1), pSer85 (1) |
