Histone H2B antibody (acLys12)

Catalog No. ABIN486135

The Rabbit Polyclonal anti-Histone H2B antibody (ABIN486135) specifically detects Histone H2B in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for Histone H2B antibody (acLys12) (ABIN486135)

Target: Histone H2B

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Histone H2B Antibody

Binding Specificity: AA 10-59, acLys12

Specificity:

Histone H2B (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at Lys12.

AcetylationH:K12 M:K12

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys12.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.473961 (NCBI Gene Symbol: H2BFS)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background:

Synonyms: Histone cluster 1, H2bb,H2B.1, H2B/f, H2BFF, H2B histone family, member F,histone 1, H2bb

NCBI Gene Symbol: H2BFS

Molecular Weight: 13 kDa

Gene ID: 54145

UniProt: P57053