Mouse anti-Human Ig (Light Chain), (Chain kappa) Antibody

Details for Product No. ABIN487319, Supplier: Log in to see
Antigen
Epitope
Light Chain, Chain kappa
545
399
290
66
25
16
13
10
7
6
5
3
2
2
1
Reactivity
Human
660
216
74
30
19
15
10
6
5
5
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
405
367
158
81
21
7
4
1
1
Clonality (Clone)
Monoclonal ()
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Clone A8B5
Isotype IgG1
No Cross-Reactivity Hamster, Sheep (Ovine), Goat, Rabbit, Guinea Pig
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein A affinity chromatography
Alternative Name Immunoglobulin
Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Research Area Secondary Antibodies, Virology
Application Notes Flow cytometry. Positive control: Daudi human cell lineImmunohistochemistry on frozen and paraffin sections. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for Mouse anti-Human Ig (Light Chain), (Chain kappa) Antibody (ABIN487319) Mouse anti-Human Ig (Light Chain), (Chain kappa) antibody
Background publications Avilés-Salas, Chanona-Vilchis, Candelaria-Hernández, Peña-Torres, Cuéllar-Mendoza: "[Plasmoblastic lymphoma initially recognized as ovarian tumor]." in: Ginecología y obstetricia de México, Vol. 80, Issue 6, pp. 430-4, 2012 (PubMed).

Di Bartolo, Hyjek, Keller, Guasparri, Deng, Sun, Chadburn, Knowles, Cesarman: "Role of defective Oct-2 and OCA-B expression in immunoglobulin production and Kaposi's sarcoma-associated herpesvirus lytic reactivation in primary effusion lymphoma." in: Journal of virology, Vol. 83, Issue 9, pp. 4308-15, 2009 (PubMed).

Scheeren, Diehl, Smit, Beaumont, Naspetti, Bende, Blom, Karube, Ohshima, van Noesel, Spits: "IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis." in: Blood, Vol. 111, Issue 9, pp. 4706-15, 2008 (PubMed).

Konig, Wennemuth, Soyer, Hoffmann, Happle, Krause: "Vulvar amyloidosis mimicking giant condylomata acuminata in a patient with multiple myeloma." in: European journal of dermatology : EJD, Vol. 9, Issue 1, pp. 29-31, 1999 (PubMed).

Diaz de Domingo, Lardo, Romero Artaza, Merelli, Carbia, Sánchez Avalos: "[Immunocytochemistry techniques for the diagnosis of hematologic neoplasms]." in: Medicina, Vol. 59, Issue 1, pp. 11-6, 1999 (PubMed).

Siddiqui, Brandriss: "The Saccharomyces cerevisiae PUT3 activator protein associates with proline-specific upstream activation sequences." in: Molecular and cellular biology, Vol. 9, Issue 11, pp. 4706-12, 1990 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).