Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody
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- Target See all Ig products
- Ig
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Binding Specificity
- Chain kappa, Light Chain
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- No Cross-Reactivity
- Hamster, Sheep (Ovine), Goat, Rabbit, Guinea Pig
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Protein A affinity chromatography
- Clone
- A8B5
- Isotype
- IgG1
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- Application Notes
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Flow cytometry. Positive control: Daudi human cell lineImmunohistochemistry on frozen and paraffin sections. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Ig
- Alternative Name
- Immunoglobulin (Ig Products)
- Background
- Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
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