OAT antibody

Antigen: OAT

Clonality: Monoclonal (AT23A2)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN487353

Additional Information

Characteristics: Synonyms: Ornithine aminotransferase mitochondrial, Ornithine--oxo-acid aminotransferase

Gene ID: 4942

Swiss-Prot: P04181

Immunogen: Recombinant human OAT (33-439aa) purified from E. coli

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1  (Matching secondary antibodies)

Clone: AT23A2

Description: OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants andmicroorganisms. Especially OAT have sex-differential expression in the mouse kidney. OATplays crucial physiological roles in amino acid metabolism. OAT shows a large structuraland mechanistic similarity to other enzymes from the subgroup III of aminotransferases,which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced prolineaccumulation.

Specificity: The antibody recognizes human and mouse OAT. Other species not tested.

Application Details

Application Notes: ELISA. Western blot (1: 250 - 1: 1000). Immunofluorescence (1: 250 - 1: 500). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4, containing 0.09% sodium azide

Storage: Store the antibody undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°Cfor longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only