A4GNT antibody (C-Term)

Catalog No. ABIN487976

The Goat Polyclonal anti-A4GNT antibody is suitable to detect A4GNT in samples from Human, Cow and Dog. It has been validated for WB and EIA.

Quick Overview for A4GNT antibody (C-Term) (ABIN487976)

Target: A4GNT (alpha-1,4-N-Acetylglucosaminyltransferase (A4GNT))

Reactivity: Human, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This A4GNT antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-A4GNT Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to A4GNT.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-PRTYRDLIKGPEGS, from the C-Terminus of the protein sequence according to NP_057245.1.

Application Details

Application Notes: Peptide ELISA: 1/8000. Western blot: Approx 40 kDa band observed in lysates of cell line K562 (calculated MW of39.0 kDa according to NP_057245.1). Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for A4GNT

Target: A4GNT (alpha-1,4-N-Acetylglucosaminyltransferase (A4GNT))

Alternative Name: A4GNT

Background: A4GNT belongs to the glycosyltransferase 32 family. It catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans and is necessary for the synthesis of type III mucin. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.Synonyms: 4-N-acetylglucosaminyltransferase, Alpha-1, Alpha4GnT

Gene ID: 51146

NCBI Accession: NP_057245

UniProt: Q9UNA3