TGFBI antibody (Internal Region)

Catalog No. ABIN488042

The Goat Polyclonal anti-TGFBI antibody is suitable to detect TGFBI in samples from Human, Mouse, Rat and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for TGFBI antibody (Internal Region) (ABIN488042)

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TGFBI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TGFBI Antibody

Binding Specificity: Internal Region

Sequence: c-QLYTDRTEKL RPE

Specificity: Recognizes TGFBI/BIGH3.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_000349.1. Genename: TGFBI

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/32000. Western Blot: 0.2-0.5 μg/mL. Approx 70 kDa band observed in Human, Mouse and RatKidney lysates (calculated MW of 74.0 kDa according to Human NP_000349.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TGFBI

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Alternative Name: TGFBI / BIGH3

Background: TGFBI, also known as BIGH3 and βig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF)-beta 1. TGFBI protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of TGFBI were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain).Synonyms: Beta ig-h3, Kerato-epithelin, RGD-CAP, RGD-containing collagen-associated protein, Transforming growth factor-beta-induced protein ig-h3

Gene ID: 7045

NCBI Accession: NP_000349

UniProt: Q15582