TGFBI antibody (Internal Region)
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- Target See all TGFBI Antibodies
- TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This TGFBI antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
- Purpose
- TGFBI
- Sequence
- QLYTDRTEKL RPE
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-QLYTDRTEKLRPE, from the internal region of the protein sequence according to NP_000349.1.
- Isotype
- IgG
- Top Product
- Discover our top product TGFBI Primary Antibody
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- Application Notes
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Immunohistochemistry: In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration: 4-6 μg/mL. This antibody has been successfully used in IHC on Rat, PMID: 26407577.
Western Blot: Approx 70 kDa band observed in Human and Rat Kidney lysates, and approx 75 kDa in Human Prostate and Human Colorectal cancer lysates (calculated MW of 74.7 kDa according to Human NP_000349.1 and 74.8 kDa according to Rat NP_446254.1). Recommend
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the golgi and plasma membrane of HeLa cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
- Alternative Name
- TGFBI (TGFBI Products)
- Synonyms
- TGFBI antibody, BIGH3 antibody, CDB1 antibody, CDG2 antibody, CDGG1 antibody, CSD antibody, CSD1 antibody, CSD2 antibody, CSD3 antibody, EBMD antibody, LCD1 antibody, 68kDa antibody, AI181842 antibody, AI747162 antibody, Beta-ig antibody, big-h3 antibody, Big-h3 antibody, MP70 antibody, MP78 antibody, hm:zeh0933 antibody, wu:fa13f05 antibody, zgc:110495 antibody, transforming growth factor beta induced antibody, transforming growth factor, beta induced antibody, transforming growth factor, beta-induced antibody, TGFBI antibody, Tgfbi antibody, tgfbi antibody
- Background
- TGFBI, transforming growth factor, beta-induced, 68 kDa, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1, RGD-containing collagen-associated protein, kerato-epithelin
- Molecular Weight
- 74.7kDa
- Gene ID
- 7045, 21810, 24511
- NCBI Accession
- NP_000349
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