ATG5 antibody (N-Term)

Catalog No. ABIN497967

This anti-ATG5 antibody is a Chicken Polyclonal antibody detecting ATG5 in WB, IHC (p) and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for ATG5 antibody (N-Term) (ABIN497967)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ATG5 Antibody

Binding Specificity: N-Term

Specificity: Recognizes APG5L/ATG5.

Purification: Affinity Chromatography purified via peptide column.

Immunogen: Chicken polyclonal ATG5 antibody was raised against a 16 amino acid peptide from near the amino terminus of Human ATG5.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA. Western Blot: ATG5 antibody can be used for the detection of ATG5 at 1-2 μg/mL. Positive Control. Mouse Spleen Tissue Lysate. Immunohistochemistry. Positive Control. Human Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: APG5L / ATG5

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5

Gene ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy