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ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (C-Term) antibody

Details for Product No. ABIN497973, Supplier: Log in to see
Antigen
  • 1810013K23Rik
  • Agp7
  • AO090001000744
  • APG7-LIKE
  • Apg7l
  • APG7L
  • ATAPG7
  • ATATG7
  • atg7
  • ATG7
  • Atg7l
  • AUTOPHAGY-RELATED 7
  • AUTOPHAGY 7
  • GSA7
  • K15I22.10
  • K15I22_10
Epitope
C-Term
29
19
15
14
13
8
7
5
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
149
44
26
Host
Rabbit
143
10
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
120
63
57
36
22
16
12
6
5
3
3
2
1
Supplier
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Immunogen APG7 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of Human APG7L/ATG7 (NP_006386).
Isotype IgG
Specificity Recognizes APG7L/ATG7 (C-term).
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Immunoaffinity Chromatography
Alternative Name APG7L / ATG7 (ATG7 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme, this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).Synonyms: APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein
Gene ID 10533
NCBI Accession NP_006386
UniProt O95352
Application Notes ELISA. Western blot: APG7 antibody can be used for the detection of APG7 at 0.5-1 μg/mL. Positive Control: Caco-2 Cell Lysate. Immunocytochemistry. Positive Control: MCF7 Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (C-Term) antibody (ABIN497973) Immunocytochemistry of ATG7 in MCF7 cells with ATG7 Antibody at 10 μg/ml.
Immunofluorescence (IF) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (C-Term) antibody (ABIN497973) Immunofluorescence of ATG7 in MCF7 cells with ATG7 Antibody at 20 µg/ml.
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (C-Term) antibody (ABIN497973) Western blot analysis of ATG7 in Caco-2 cell lysate with ATG7 Antibody at (A) 0.5, (B...
Background publications Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004

Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000

Mizushima, Noda, Yoshimori, Tanaka, Ishii, George, Klionsky, Ohsumi, Ohsumi: "A protein conjugation system essential for autophagy." in: Nature, Vol. 395, Issue 6700, pp. 395-8, 1998

Kisen, Tessitore, Costelli, Gordon, Schwarze, Baccino, Seglen: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994