PRPF31 antibody (C-Term)

Catalog No. ABIN498020

The Goat Polyclonal anti-PRPF31 antibody is suitable to detect PRPF31 in samples from Human and Dog. It has been validated for WB and EIA.

Quick Overview for PRPF31 antibody (C-Term) (ABIN498020)

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PRPF31 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PRPF31 Antibody

Binding Specificity: C-Term

Sequence: C-LKVKGEKSGL MST

Specificity: Recognizes RP11/PRPF31 (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_056444.3. Genename: PRPF31

Application Details

Application Notes: ELISA: Detection limit dilution: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 60 kDa band observed in lysates of cell line A431(calculated MW of 55.0 kDa according to NP_056444.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRPF31

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Alternative Name: PRPF31

Background: PRPF31 is involved in pre-mRNA splicing. It is required for U4/U6.U5 tri-snRNP formation.Synonyms: NY-BR-99, PRP31, Pre-mRNA-processing factor 31, Serologically defined breast cancer antigen NY-BR-99, U4/U6 small nuclear ribonucleoprotein Prp31

Gene ID: 26121

NCBI Accession: NP_056444

UniProt: Q8WWY3

Pathways: Ribonucleoprotein Complex Subunit Organization