CPT2 antibody (Internal Region)

Catalog No. ABIN498063

Product Details anti-CPT2 Antibody

Target: CPT2 (Carnitine Palmitoyltransferase 2 (CPT2))

Binding Specificity: AA 141-154, Internal Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CPT2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-PKSEYNDQLT RATN

Specificity: Recognizes Carnitine O-palmitoyltransferase CPT2

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of Human CPT2 according to NP_000089.1. Genename: CPT2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 1-3 μg/mL. Detects a band of Approx 70 kDa in Human and Mouse Liver lysates. An additional band of unknown identity was also consistently observed at 20 kDa. Thisband was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CPT2

Target: CPT2 (Carnitine Palmitoyltransferase 2 (CPT2))

Alternative Name: CPT2 (CPT2 Products)

Synonyms: CPT1 antibody, CPTASE antibody, IIAE4 antibody, AI323697 antibody, CPTII antibody, cg2107 antibody, wu:fa03e08 antibody, wu:fb54a02 antibody, zgc:101627 antibody, carnitine palmitoyltransferase 2 antibody, carnitine O-palmitoyltransferase 2, mitochondrial antibody, carnitine palmitoyltransferase 2 S homeolog antibody, CPT2 antibody, LOC100214547 antibody, Cpt2 antibody, cpt2.S antibody, cpt2 antibody

Background: Carnitine palmitoyltransferase II (CPT2) together with carnitine palmitoyltransferase I oxidizes long-chain fatty acids in the mitochondria. Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency (CPT2D) also known as CPT-II deficiency or CPT2 deficiency. CPT2D is an autosomal recessive disorder characterized by recurrent myoglobinuria, episodes of muscle pain, stiffness, and rhabdomyolysis. These symptoms are triggered by prolonged exercise, fasting or viral infection and patients are usually young adults. In addition to this classical, late-onset, muscular type, a hepatic or hepatocardiomuscular form has been reported in infants. Clinical pictures in these children or neonates include hypoketotic hypoglycemia, liver dysfunction, cardiomyopathy and sudden death.Synonyms: CPT II, Carnitine O-palmitoyltransferase 2, Carnitine palmitoyltransferase II, mitochondrial

Gene ID: 1376

NCBI Accession: NP_000089

UniProt: P23786

Pathways: Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process