ALDH2 antibody (Internal Region)

Catalog No. ABIN498080

The Goat Polyclonal anti-ALDH2 antibody is suitable to detect ALDH2 in samples from Human, Rat, Mouse, Cow, Dog and Pig. It has been validated for WB and EIA.

Quick Overview for ALDH2 antibody (Internal Region) (ABIN498080)

Target: ALDH2 (Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))

Reactivity: Human, Rat, Mouse, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDH2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ALDH2 Antibody

Binding Specificity: Internal Region

Sequence: C-DETQFKKILG YIN

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000681.2. Genename: ALDH2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.03-0.1 μg/mL. Detects a band of Approx 50 kDa in Human, Mouse and Rat Liver lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human, Mouse, Rat. Expected from sequence similarity: Canine (Dog), Bovine, Pig. Add. Information: Calculated Molecular Weight: 56.0 kDa (Human NP_000681.2) and 56.0 kDa (MouseNP_033786.1)

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALDH2

Target: ALDH2 (Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))

Alternative Name: ALDH2

Background: ALDH2 (Aldehyde Dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations.Synonyms: ALDH class 2, ALDH-E2, ALDHI, ALDM, Aldehyde dehydrogenase mitochondrial

Gene ID: 217

NCBI Accession: NP_000681

UniProt: P05091