NDUFS1 antibody (Internal Region)

Catalog No. ABIN498087

The Goat Polyclonal anti-NDUFS1 antibody (ABIN498087) specifically detects NDUFS1 in WB and EIA. The antibody is reactive with Human, Rat, Mouse, Cow, Rabbit and Dog samples.

Quick Overview for NDUFS1 antibody (Internal Region) (ABIN498087)

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NDUFS1 Antibody

Binding Specificity: Internal Region

Sequence: C-TEKSATYVNT EGR

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine and RabbitSpecies reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_004997.4. Genename: NDUFS1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 80 kDa in Human Heart and Mouse Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human, Mouse. Expected from sequence similarity: Rat, Canine (Dog), Bovine and RabbitAdd. Information: Calculated Molecular Weight: 79.0 kDa according to NP_004997.4

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDUFS1

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Alternative Name: NDUFS1

Background: NDUFS1 belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. Ndufs1 has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in Ndufs1 gene are associated with complex I deficiency.Synonyms: CI-75kD, Complex I-75kD, NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene ID: 4719

NCBI Accession: NP_004997

UniProt: P28331