NDUFS2 antibody (Internal Region)

Catalog No. ABIN498088

Product Details anti-NDUFS2 Antibody

Target: NDUFS2 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase) (NDUFS2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-SRGVRQWQPD VE

Specificity: This antibody is expected to recognize both reported isoforms (NP_004541.1, NP_001159631.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine, Porcine and Rabbit.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_004541.1, NP_001159631.1. Genename: NDUFS2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.1-0.3 μg/mL. Detects a band of Approx 48 kDa in Human, Mouse and Rat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDUFS2

Target: NDUFS2 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase) (NDUFS2))

Alternative Name: NDUFS2 (NDUFS2 Products)

Synonyms: AL033311 antibody, CI-49 antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 2 antibody, NADH:ubiquinone oxidoreductase core subunit S2 antibody, Ndufs2 antibody, NDUFS2 antibody

Background: NDUFS2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria.Synonyms: CI-49kD, Complex I-49kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial

Molecular Weight: 51.9kDa according to NP_001159631.1)

Gene ID: 4720

NCBI Accession: NP_004541

UniProt: O75306