ATP1A1 antibody

Catalog No. ABIN498101

Product Details anti-ATP1A1 Antibody

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody antibody detects endogenous levels of Na(+)/K(+)-ATPase alpha1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blots: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200. Immunofluorescence: 1/100approx. 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATP1A1 (ATP1A1 Products)

Synonyms: Atpa-1 antibody, BC010319 antibody, Nkaa1b antibody, K-ATPase antibody, Na+K+ATPase antibody, atp1a1 antibody, atp1a1a.1 antibody, atp1a1l1 antibody, atp[a]1B1 antibody, cb70 antibody, wu:fa03c03 antibody, wu:fb92h10 antibody, atp1a1l5 antibody, atp[a]1B2 antibody, cb707 antibody, wu:fj34g01 antibody, zgc:92351 antibody, ATPase Na+/K+ transporting subunit alpha 1 antibody, ATPase, Na+/K+ transporting, alpha 1 polypeptide antibody, ATPase Na+/K+ transporting subunit alpha 1 L homeolog antibody, ATPase, Na+/K+ transporting, alpha 1a polypeptide, tandem duplicate 1 antibody, ATPase, Na+/K+ transporting, alpha 1b polypeptide antibody, ATP1A1 antibody, Atp1a1 antibody, atp1a1.L antibody, atp1a1a.1 antibody, atp1a1b antibody

Background: The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.Synonyms: Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump ATPase subunit alpha-1, Sodium/potassium-transporting ATPase subunit alpha-1

Molecular Weight: approx. 112 kDa

Gene ID: 476

NCBI Accession: NP_000692

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process