Histone H1 antibody
-
- Target See all Histone H1 (H1F0) Antibodies
- Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Histone H1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of Histone H1 protein.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity chromatography using epitope-specific immunogen
- Purity
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product H1F0 Primary Antibody
-
-
- Application Notes
-
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
-
- Target
- Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))
- Alternative Name
- Histone H1.0 (H1F0 Products)
- Synonyms
- H5 antibody, h10 antibody, h1fv antibody, fb23b02 antibody, wu:fb23b02 antibody, zgc:65861 antibody, H1F0 antibody, H1fv antibody, H10 antibody, H1FV antibody, D130017D06Rik antibody, H1(0) antibody, h1(0)-1 antibody, h1f0 antibody, H1 antibody, H1 histone family member 0 antibody, H1 histone family, member 0 antibody, histone H1.0 antibody, H1 histone family member 0 S homeolog antibody, H1 histone family member 0 L homeolog antibody, histone I antibody, h1f0 antibody, H1F0 antibody, LOC698693 antibody, H1f0 antibody, h1f0.S antibody, h1f0.L antibody, his1 antibody
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.The C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo. The C-terminal region also shows the most differences in aa sequence between the H1 histone variants H1.0/1/2/3/4/5.Synonyms: H1F0, H1FV, Histone H1', Histone H1(0)
- Molecular Weight
- approx. 35 kDa
- Gene ID
- 3005
- NCBI Accession
- NP_005309
- UniProt
- P07305
-