ACLY antibody
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- Target See all ACLY Antibodies
- ACLY (ATP Citrate Lyase (ACLY))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ACLY antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- This antibody detects endogenous levels of ATP-citrate synthase protein.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product ACLY Primary Antibody
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- Application Notes
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Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2 with 0.05 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- ACLY (ATP Citrate Lyase (ACLY))
- Alternative Name
- ATP-Citrate Synthase (ACLY Products)
- Synonyms
- ACLY antibody, DDBDRAFT_0205389 antibody, DDBDRAFT_0235360 antibody, DDB_0205389 antibody, DDB_0235360 antibody, ACL antibody, BcDNA:LD21334 antibody, CG8322 antibody, DmATPCL antibody, Dmel\\CG8322 antibody, anon-WO0140519.179 antibody, dATPCL antibody, l(2)01466 antibody, l(2)k09217 antibody, n(2)k09217 antibody, ATPCL antibody, CLATP antibody, A730098H14Rik antibody, AW538652 antibody, Clatp antibody, acly antibody, cb722 antibody, zgc:92008 antibody, ATP citrate lyase antibody, ATP citrate lyase S homeolog antibody, ATP-citrate synthase antibody, citrate synthase, mitochondrial antibody, atp-citrate synthase antibody, ATP citrate lyase a antibody, ACLY antibody, acly.S antibody, LOC587157 antibody, acly antibody, ATPCL antibody, LOC5564509 antibody, CpipJ_CPIJ010291 antibody, Bm1_26245 antibody, ACL antibody, Acly antibody, aclya antibody
- Background
- ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL.Synonyms: ACLY, ATP citrate (pro-S-)-lyase, Citrate cleavage enzyme
- Molecular Weight
- approx. 120 kDa
- Gene ID
- 47
- NCBI Accession
- NP_001087
- UniProt
- P53396
- Pathways
- Warburg Effect
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