STAM2 antibody

Catalog No. ABIN498238

This Rabbit Polyclonal antibody specifically detects STAM2 in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for STAM2 antibody (ABIN498238)

Target: STAM2 (Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 2 (STAM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STAM2 Antibody

Specificity: This antibody detects endogenous levels of STAM2 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STAM2

Target: STAM2 (Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 2 (STAM2))

Alternative Name: STAM2

Background: Cytokine stimulation of the IL-2 receptor leads to the tyrosine phosphorylation of a number of cellular proteins and to the induction of various transcription factors including c-Fos and c-Myc. The signal transducing adapter molecule, STAM, is speculated to play a role in c-Myc induction by various cytokines. STAM contains an SH3 (Src homology 3) motif as well as an immunoreceptor tyrosine-based activation (ITAM) motif, both of which appear to be required for c-Myc induction in response to IL-2 and GM-CSF. STAM associates with JAK3 and JAK2 via its ITAM region, and it is tyrosine phosphorylated by JAK3 and JAK2 after stimulation with IL-2 and GM-CSF, respectively. STAM2, also known as Hbp, is a protein that is highly related to STAM. Similar to STAM, STAM2 functions downstream of JAK kinases and can be phosphorylated in response to cytokines. Due to alternative splicing events, two isoforms of STAM2 exist, namely STAM2A and STAM2B.Synonyms: HBP, Hrs-binding protein, STAM-2, STAM2A, STAM2B, Signal transducing adapter molecule 2

Molecular Weight: approx. 58 kDa

Gene ID: 10254

NCBI Accession: NP_005834

UniProt: O75886

Pathways: EGFR Signaling Pathway, EGFR Downregulation