STAM2 antibody (Internal Region)

Catalog No. ABIN185613

The Goat Polyclonal anti-STAM2 antibody (ABIN185613) specifically detects STAM2 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for STAM2 antibody (Internal Region) (ABIN185613)

Target: STAM2 (Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 2 (STAM2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STAM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-STAM2 Antibody

Binding Specificity: Internal Region

Purpose: STAM2

Sequence: QSIDPTDSKP DSQD

Specificity: No cross-reaction expected with STAM1.

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QSIDPTDSKPDSQD, from the internal region of the protein sequence according to NP_005834.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Spleen lysates and in preliminary testing of HeLa cell lysate (calculated MW of 58.2 kDa according to NP_005834.3). This molecular weight is routinely observed by other sources. Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for STAM2

Target: STAM2 (Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 2 (STAM2))

Alternative Name: STAM2

Background: STAM2, signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 , HGNC:11358, DKFZp564C047, Hbp, STAM2A, STAM2B , STAM-like protein containing SH3 and ITAM domains 2, signal transducing adaptor molecule 2

Gene ID: 10254, 56324

NCBI Accession: NP_005834

Pathways: EGFR Signaling Pathway, EGFR Downregulation