DMPK antibody

Catalog No. ABIN498331

Product Details anti-DMPK Antibody

Target: DMPK (Dystrophia Myotonica-Protein Kinase (DMPK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMPK antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of DMPK protein. (region surrounding Arg30)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for DMPK

Target: DMPK (Dystrophia Myotonica-Protein Kinase (DMPK))

Alternative Name: DMPK (DMPK Products)

Synonyms: DMPK antibody, DM antibody, DM1 antibody, DM1PK antibody, DMK antibody, MDPK antibody, MT-PK antibody, Dm15 antibody, DM1 protein kinase antibody, dystrophia myotonica-protein kinase antibody, DMPK antibody, Dmpk antibody

Background: Myotonic dystrophy protein kinase (DMPK) is a ulti-domain protein kinase found in muscle that is activated in response to G protein second messengers and proteolysis. DMPK is implicated in myotonic muscular dystrophy (DM), an autosomal dominant-inherited disorder that predominately affects skeletal and cardiac muscle and causes defects in cardiac conduction. DM arises through expansion of CTG repeats in the 3'-UTR of the DMPK gene. Mutant DMPK transcripts with an extended region of CUG repeats are retained in the nucleus. These transcripts also influence the expression of the DM locus-associated homeodomain protein (DMAHP)/SIX5, to mediate in part the DM phenotype. Other substrates for DMPK include myogenin, Ltype calcium channels, and Phospholemman (PLM).Synonyms: DM-kinase, MDPK, MT-PK, Myotonic dystrophy protein kinase, Myotonin-protein kinase

Molecular Weight: approx. 70 kDa

Gene ID: 1760

NCBI Accession: NP_001075029

UniProt: Q09013

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development