MAPK15 antibody

Catalog No. ABIN498400

This Rabbit Polyclonal antibody specifically detects MAPK15 in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for MAPK15 antibody (ABIN498400)

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAPK15 Antibody

Specificity: This antibody detects endogenous levels of ERK8 protein. (region surrounding Asp171)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH 7.2, 0,05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MAPK15

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Alternative Name: MAPK15

Background: The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.Synonyms: ERK8, Extracellular signal-regulated kinase 8, Mitogen-activated protein kinase 15

Molecular Weight: approx. 60 kDa

Gene ID: 225689

NCBI Accession: NP_620590

UniProt: Q8TD08