IL12A antibody

Catalog No. ABIN498511

This Rabbit Polyclonal antibody specifically detects IL12A in IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IL12A antibody (ABIN498511)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL12A Antibody

Specificity: This antibody detects endogenous levels of IL-12A protein. (region surrounding Thr65)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Interleukin-12 alpha / IL12A

Background: IL-12 is secreted by macrophages and human B-lymphoblastoid cells in response to antigenic stimulation. This IL is responsible for the differentiation of naive CD4+ T cells into type 1 helper T cells that produce interferon-γ (IFN-γ). It also activates production of tumor necrosis factor α (TNFα) from T and natural killer (NK) cells, and it inhibits IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity since the production of IFN-γ increases the production of inducible protein-10 (IP-10). IL-12 p40 represents a subunit of IL-12 that is induced by several pathogens. IL-12 p70 is a subunit of IL-12 that is controlled through both the p35 and p40 genes in a cell type-specific fashion.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

Molecular Weight: approx. 25 kDa

Gene ID: 3592

NCBI Accession: NP_000873

UniProt: P29459

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation