E2F5 antibody

Catalog No. ABIN498519

The Rabbit Polyclonal anti-E2F5 antibody (ABIN498519) specifically detects E2F5 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for E2F5 antibody (ABIN498519)

Target: E2F5

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-E2F5 Antibody

Specificity: This antibody detects endogenous levels of E2F-5 protein. (region surrounding Leu142)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for E2F5

Target: E2F5

Alternative Name: E2F5

Background: The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an Synonyms: E2F transcription factor 5, E2F-5, Transcription factor E2F5, p130-binding

Molecular Weight: approx. 36 kDa

Gene ID: 1875

NCBI Accession: NP_001077057

UniProt: Q15329