E2F5 antibody (N-Term)

Catalog No. ABIN2792655

Product Details anti-E2F5 Antibody

Target: E2F5

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KAEIEDLELK ERELDQQKLL LQQSIKNVMD DSINNRFSYV THEDICNCFN

Cross-Reactivity: Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 84%

Characteristics: This is a rabbit polyclonal antibody against E2F5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human E2F5

Application Details

Application Notes: WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 346 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for E2F5

Target: E2F5

Alternative Name: E2F5 (E2F5 Products)

Synonyms: AU024671 antibody, E2F-5 antibody, E2f-5 antibody, E2F4 antibody, cb735 antibody, zgc:111879 antibody, E2F transcription factor 5 antibody, E2f5 antibody, e2f5 antibody, E2F5 antibody

Background: E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
Alias Symbols: E2F-5
Protein Interaction Partner: RBL2, UBC, KMT2D, BRCC3, SMAD3, EP300, XPO1, CREBBP, TFDP1, RBL1, BIRC5, MYC, MT1G,
Protein Size: 346

Molecular Weight: 38 kDa

Gene ID: 1875

NCBI Accession: NM_001951, NP_001942

UniProt: Q15329