IL5RA antibody

Catalog No. ABIN498570

The Rabbit Polyclonal anti-IL5RA antibody (ABIN498570) specifically detects IL5RA in IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for IL5RA antibody (ABIN498570)

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL5RA antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL5RA Antibody

Specificity: This antibody detects endogenous levels of IL-5Ralpha protein. (region surrounding Lys68)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL5RA

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Alternative Name: CD125 / IL5RA

Background: Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membraneproximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear protooncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2. Alternative splicing of the IL-5Ralpha gene produces several isoforms, including a membraneanchored isoform and a soluble isoform. The soluble isoform competes with IL-5 for binding to IL-5R and inhibits IL-5-mediated receptor activation and inflammatory mediator production, and, therefore, may be useful in treating asthma.Synonyms: IL-5R-alpha, IL5 Receptor alpha, Interleukin-5 receptor subunit alpha

Gene ID: 3568

NCBI Accession: NP_000555

UniProt: Q01344

Pathways: JAK-STAT Signaling