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Paxillin antibody (pTyr31)
PXN
Reactivity: Human, Mouse, Rat
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-Paxillin Antibody
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Target
See all Paxillin (PXN) Antibodies
Paxillin (PXN)
Binding Specificity
All epitopes for Paxillin antibodies
pTyr31
Reactivity
All reactivities for Paxillin antibodies
Human, Mouse, Rat
Host
All hosts for Paxillin antibodies
Rabbit
Clonality
All clonalities for Paxillin antibodies
Polyclonal
Conjugate
All conjugates for Paxillin antibodies
This Paxillin antibody is un-conjugated
Application
All applications for Paxillin antibodies
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody detects endogenous levels of p-Paxillin protein, only when phosphorylated at Tyr31.
Cross-Reactivity (Details)
Species reactivity (expected):Mouse and Rat. Species reactivity (tested):Human.
Purification
Affinity chromatography
Purity
> 95 % by SDS-PAGE
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Discover our top product PXN Primary Antibody
Alternatives
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Application Details
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Application Notes
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
1,0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for Paxillin
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Target
Paxillin (PXN)
Alternative Name
Paxillin / PXN (PXN Products )
Synonyms
AW108311 antibody, AW123232 antibody, Pax antibody, CG18061 antibody, CG18576 antibody, CG31794 antibody, CT40481 antibody, CT42454 antibody, DPaxillin antibody, DPxn antibody, DPxn37 antibody, Dmel\\CG31794 antibody, Dpax antibody, DpaxA antibody, PDLP antibody, dPax antibody, pax antibody, wu:fw71f12 antibody, PXN antibody, LOC100220858 antibody, paxillin antibody, Paxillin antibody, paxillin S homeolog antibody, paxillin a antibody, PXN antibody, Pxn antibody, Pax antibody, pxn.S antibody, pxna antibody, LOC696336 antibody, pxn antibody
Background
The effects of some oncogenes, growth factors and neuropeptides are mediated by tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin cytoskeletal proteins. A rapid increase in tyrosine phosphorylation of paxillin, FAK and Crk-associated substrate (CAS) are prominent early events triggered by many G protein-coupled receptors. In addition to G protein-coupled receptors, Angiotensin IV (Ang IV), protein kinase C and other proteins can also mediate the tyrosine phosphorylation of paxillin. Paxillin must bind FAK for maximal phosphorylation in response to cell adhesion. FAK may function to direct tyrosine phosphorylation of paxillin in the process of transformation by the Src oncogene.Synonyms: FLJ16691
Molecular Weight
approx. 68 kDa
Gene ID
5829
NCBI Accession
NP_001074324
UniProt
P49023
Pathways
MAPK Signaling , EGFR Signaling Pathway , Response to Growth Hormone Stimulus , Cell-Cell Junction Organization , Maintenance of Protein Location , CXCR4-mediated Signaling Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling
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