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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody

Antigen

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)
Synonyms SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70
Epitope
Alternatives

pTyr493
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN498719
Quantity 100 µg  (1,0 mg/ml)  (Variants)
Price 340.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase
Alternative name ZAP70
Gene ID 7535
Swiss-Prot P43403
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Description The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), whichis a multisubunit complex assembled from at least six different genes. The TCR subunitsinclude the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and azeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds tothe phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCRzeta chain through two Src homology (SH2) domains. This binding results in thephosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylationon Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.
Specificity P-ZAP-70 (pTyr493) pAb detects endogenous levels of p-ZAP-70 protein. Species: Human, Mouse, Rat. Others not tested.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1,0 mg/ml
Purification Aff - Purified
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
Hosts Rabbit (99), Mouse (42)
Reactivities Human (136), Mouse (Murine) (65), Rat (Rattus) (59), Cow (Bovine) (18), Pig (Porcine) (18), Dog (Canine) (17), Horse (Equine) (16), Rabbit (16), Chicken (15), Guinea Pig (15)
Applications Western Blotting (WB) (78), Flow Cytometry (FACS) (37), Immunohistochemistry (IHC) (33), ELISA (31), Immunofluorescence (IF) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Enzyme Immunoassay (EIA) (12), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (6), FITC (6), PE (3), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
Epitopes pTyr319 (10), pTyr493 (7), Ab-319 (5), Ab-493 (5), C-Term (5), Center (3), Ab-292 (1), Ab-315 (1), pTyr292 (1), pTyr315 (1), pTyr492 (1)