GATA1 antibody (pSer142)

Catalog No. ABIN498843

The Rabbit Polyclonal anti-GATA1 antibody (ABIN498843) specifically detects GATA1 in IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GATA1 antibody (pSer142) (ABIN498843)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GATA1 Antibody

Binding Specificity: pSer142

Specificity: This antibody detects endogenous levels of p-GATA-1 protein only when phosphorylated at Ser142.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA1

Background: Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroidspecific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4, expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart.Synonyms: ERYF1, Eryf1, Erythroid transcription factor, GATA-1, GATA-binding factor 1, GF-1, GF1, NF-E1 DNA-binding protein

Molecular Weight: approx. 43 kDa

Gene ID: 2623

NCBI Accession: NP_002040

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin